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The sitters are Gabriel Vendramin, the collector of works of art, Andrea Vendramin (died 1547), and the latter's seven sons. The reliquary of the True Cross on the altar still exists. It was presented to an earlier Andrea Vendramin, Guardian of the Scuola di S. Giovanni Evangelista, Venice, on behalf of this confraternity in 1369. On one occasion he is said to have rescued it from a canal.

The painting of the children, with the exception of the boy holding a puppy on the right, must be by the artist's workshop.

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